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遗传图谱的构建及整合是开展花生分子育种研究的基础,利用多个作图群体整合遗传图谱是解决图谱标记密度低的有效途径。本研究采用基于锚定SSR标记的作图策略,构建3个F_2群体3张遗传连锁图,利用Join Map 3.0软件整合图谱,获得一张包含20个连锁群、792个位点、总遗传距离为2079.50 c M,标记间平均距离为2.63 c M的整合图谱,各连锁群标记数在20~66个之间,遗传距离在59.10~175.80 c M之间。将3个分离群体中检测到的与荚果及种子大小相关的QTL区段与整合连锁图的标记比较发现,各群体中检测到的位于各染色体上的QTL在整合图谱中都能出现,有些QTL标记区间在整合图谱中存在更多的标记,为今后利用这些标记进行精细定位奠定了基础。
The construction and integration of genetic maps are the basis for the molecular breeding of peanut. Using multiple mapping populations to integrate genetic maps is an effective way to solve the problem of low density of map markers. In this study, a mapping strategy based on anchored SSR markers was used to construct 3 genetic linkage maps of 3 F_2 populations. Using Join Map 3.0 software to integrate the map, a map of 20 linkage groups and 792 loci was obtained. The total genetic distance was 2079.50 cM, the average distance between markers was 2.63 cM, the number of linkage group markers was between 20 and 66, and the genetic distance was between 59.10 and 175.80 cM. Comparing the QTL segments related to pod and seed size detected in the three segregating populations with the markers in the integrated linkage map, it was found that QTLs detected in each population could be found in all the chromosomes. Some QTLs The marker interval has more markers in the integration map, which lays the foundation for fine mapping using these markers in the future.