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目的探讨酰化低分子量肝素(ALMWH)对人乳腺癌细胞MDA-MB-231增殖的抑制及对人乳腺癌细胞MDA-MB-231裸鼠肺转移抑制作用。方法制备ALMWH。细胞实验设空白组、紫杉醇组、低分子量肝素组及不同浓度ALMWH组。药物处理人乳腺癌细胞MDA-MB-231,于24、48、和72 h后收集细胞进行直接活细胞计数。动物实验设模型组、紫杉醇组、低分子量肝素组及不同浓度ALMWH组。经裸鼠尾静脉注射人乳腺癌细胞MDA-MB-231并间日给药20 d检查。结果 ALMWH能显著抑制人乳腺癌细胞MDA-MB-231的增殖,其抑制作用强于等剂量低分子量肝素,具有剂量和时间依赖性;ALMWH有抑制小鼠人乳腺癌细胞MDA-MB-231肺转移能力,明显减少肺部的转移灶;ALMWH各组小鼠肺部瘤灶数目与模型组比较显著减少且瘤灶体积也明显变小,作用明显优于低分子量肝素组。结论 ALMWH具有较高的抗肿瘤增殖及肺转移活性,可望成为一个新型的抗肿瘤药。
Objective To investigate the inhibitory effect of acylated low molecular weight heparin (ALMWH) on the proliferation of human breast cancer cell line MDA-MB-231 and its inhibitory effect on lung metastasis of human breast cancer cell line MDA-MB-231. Method to prepare ALMWH. Cell experiments set the blank group, paclitaxel group, low molecular weight heparin group and different concentrations of ALMWH group. Human breast cancer cells MDA-MB-231 were drug-treated and cells were harvested at 24, 48, and 72 h for direct viable cell counting. Animal model group, paclitaxel group, low molecular weight heparin group and different concentrations of ALMWH group. The human breast cancer cells MDA-MB-231 were injected through the caudal vein of nude mice and dosed daily for 20 days. Results ALMWH could significantly inhibit the proliferation of human breast cancer cell line MDA-MB-231, its inhibitory effect was stronger than that of low-molecular-weight heparin with dose-dependent and time-dependent manner. ALMWH inhibited the growth of mouse breast cancer cell line MDA-MB-231 Metastasis ability, significantly reduce the lung metastasis; ALMWH mice lung tumor number and the model group was significantly reduced and tumor volume was significantly smaller, the role was significantly better than the low molecular weight heparin group. Conclusion ALMWH has a high anti-tumor proliferation and lung metastasis activity, is expected to become a new type of antineoplastic agents.