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目的建立测定丹酚及其滴丸中6种主要丹酚酸含量的方法。方法采用HPLC法,Unitary C18色谱柱,乙腈-0.05%磷酸水为流动相梯度洗脱,检测波长286 nm,流速为1.1 ml·min-1,柱温为35℃。结果该色谱条件下,6种酚酸类成分可完全分离。丹参素、原儿茶醛、迷迭香酸、紫草酸、丹酚酸B和丹酚酸A的线性范围分别为2.07~20.66μg·ml-1(r=0.9998)、2.53~25.30μg·ml-1(r=0.9999)、8.12~40.60μg·ml-1(r=0.9999)、11.24~56.20μg·ml-1(r=0.9999)、99.60~996.00μg·ml-1(r=0.9999)、1.00~10.02μg·ml-1(r=0.9998),6种成分的平均回收率为99.24%~101.55%,RSD均小于2%。结论该测定方法准确度高,重复性好,专属性强,可同时测定丹酚及其滴丸中6种丹酚酸的含量。
Objective To establish a method for the determination of six major salvianolic acids in Dan and its dropping pills. Methods The HPLC method, Unitary C18 column and acetonitrile-0.05% phosphoric acid water were used as mobile phase. The detection wavelength was 286 nm, the flow rate was 1.1 ml · min-1 and the column temperature was 35 ℃. Results Under the chromatographic conditions, six kinds of phenolic acids can be completely separated. The linear ranges of danshensu, protocatechuic acid, rosmarinic acid, lithospermic acid, salvianolic acid B and salvianolic acid A were 2.07-20.66μg · ml-1 (r = 0.9998), 2.53-25.50μg · ml (R = 0.9999), 99.60 ~ 996.00 μg · ml-1 (r = 0.9999), and 1.00 ~ 10.02μg · ml-1 (r = 0.9998). The average recoveries of the six components ranged from 99.24% to 101.55% with RSD less than 2%. Conclusion The method has the advantages of high accuracy, good reproducibility and specificity. It can simultaneously determine the content of six kinds of salvianolic acid in the decoction of Dan and its drops.