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以大肠杆菌为接种体,葡萄糖为基质,在1000Ω恒外阻下生成电活性生物膜,研究了生物膜的形成对电池电化学行为的影响.应用循环伏安、阻抗测试、极化分析、输出功率和阳极电势来考察其电化学表现.研究结果表明,随着生物膜完全成熟,阳极极化电阻减小66.5％,阳极电势逐渐降低,最大输出功率密度增加260％.“,”Electroactive biofilms were formed on carbon paper under constant external resistance of 1 000 Ω using Escherichia coli as inoculum and glucose as substrate.In this paper,the performance of the biofilm growth of the microorganism directly on the anode was studied.To form a mature biofilm,five fed-batch cycles were repeated with every period of 1 day.The electrochemical characterization of the microbial fuel cell was evaluated using cyclic voltammetry ( CV),electrochemical impedance spectroscopy (EIS) and polarization behavior.It was evident that the enhanced MFC performance was associated with the development of the biofilm.With formation of the mature anode biofilm,the anode polarization resistance was decreased by 66.5％,the anode potential also gradually decreased,while peak output power density was enhanced over 260％.