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目的:探讨抗氧化剂N-乙酰半胱氨酸(NAC)和川芎嗪对博莱霉素所致小鼠肺间质纤维化的治疗作用及机制。方法:博莱霉素(BLM)制成小鼠肺间质纤维化模型。各组动物处死后提取肺组织和支气管肺泡灌洗液(BALF),进行病理半定量分析,检测谷胱甘肽(GSH)和丙二醛(MDA)含量、BALF中转化生长因子-β(TGF-β)水平。结果:NAC和川芎嗪治疗组肺泡炎、纤维化病变均较模型组减轻,7 d时肺组织GSH量较模型组高(P<0.05),MDA含量较模型组低(P<0.05),7、14 d时TGF-β蛋白水平较模型组低(P<0.05)。结论:NAC和川芎嗪可提高肺组织GSH含量,降低MDA生成,使肺泡炎和肺纤维化病变减轻。NAC和川芎嗪通过抗氧化作用,减少TGF-β产生,是其治疗肺纤维化的机制之一。
Objective: To investigate the therapeutic effect and mechanism of antioxidants N-acetylcysteine (NAC) and tetramethylpyrazine on bleomycin induced pulmonary interstitial fibrosis in mice. Methods: Bleomycin (BLM) was used to make mouse pulmonary interstitial fibrosis model. Lung tissues and bronchoalveolar lavage fluid (BALF) were extracted after sacrifice in each group. Semi-quantitative analysis of pathological changes was performed. The levels of glutathione (GSH) and malondialdehyde (MDA) were measured. The levels of transforming growth factor- -β) levels. Results: The alveolitis and fibrosis in the NAC group and the Ligustrazine group were both alleviated compared with the model group. At 7 d, the GSH level in lung tissue was higher (P <0.05) and MDA content was lower (P <0.05) The level of TGF-βprotein was lower on the 14th day than that of the model group (P <0.05). Conclusion: NAC and ligustrazine can increase the content of GSH in lung tissue, reduce the production of MDA, and alleviate the alveolitis and pulmonary fibrosis. NAC and tetramethylpyrazine through anti-oxidation, reduce TGF-β production, is one of its mechanisms for the treatment of pulmonary fibrosis.