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目的探讨人群中HBcAg、HBV血清标志物和HBV DNA检出情况及三者关系,分析它们在乙型肝炎病人中存在的原因及临床价值,为临床提供乙肝病毒传染性及诊断的实验室依据。方法用放射免疫法检测1 460例标本的血清标志物,同时对其中133例标本用聚合酶链反应(PCR)法检测HBVDNA。结果421例HBsAg阳性中HBcAg阳性率为86.94%,98例HBeAg阳性中HBcAg阳性率为65.31%。在同时检测HBcAg和HBV DNA的133例各种感染模式中,HBcAg与HBV DNA阳性率相似,差异无统计学意义(P>0.05)。结论HBcAg与具有病毒活动性复制意义的指标HBV DNA有良好的一致性。HBcAg与HBV DNA可以反映HBV感染与复制,尤其可反映HBsAg阴性或HBeAg阴性的乙肝患者仍存在的病毒复制。
Objective To investigate the detection of HBcAg, HBV serum markers and HBV DNA in the population and the relationship among them, analyze their causes and clinical value in patients with hepatitis B, and provide laboratory basis for clinical diagnosis and diagnosis of hepatitis B virus. Methods Serum markers of 1 460 samples were detected by radioimmunoassay, and HBVDNA was detected by polymerase chain reaction (PCR) in 133 of them. Results The positive rate of HBcAg was 86.94% in 421 HBsAg positive cases and 65.31% in 98 HBeAg positive cases. The positive rates of HBcAg and HBV DNA in 133 cases of all kinds of infection with simultaneous detection of HBcAg and HBV DNA were not statistically different (P> 0.05). Conclusions HBcAg has good consistency with HBV DNA which has the significance of active viral replication. HBcAg and HBV DNA can reflect HBV infection and replication, in particular, can reflect the HBsAg-negative or HBeAg-negative hepatitis B virus remains in the virus replication.