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利用全细胞膜片钳技术 ,采用胶原酶B急性分离的大鼠心室肌细胞 ,研究了牛黄酸和二甲基 -氨氯吡咪对心肌细胞膜Na+/Ca2 +交换电流的影响。结果表明 ,10 μmol,2 0 μmol和 4 0 μmol的二甲基 -氨氯吡咪可使Ni2 +敏感电流浓度依赖性地增加 ;膜电位为 +5 0mV时分别增加 ( 2 7± 7) % ,( 12 1± 4 3 ) % ,和 ( 173± 68) % ;膜电位为 - 10 0mV时分别增加 ( 110±5 2 ) % ,( 2 2 1± 88) %和 ( 2 81± 80 ) %。牛黄酸对Na+/Ca2 +交换的内向和外向电流均无影响
Whole cell patch clamp technique was used to study the effect of taurine and dimethyl-amiloride on the Na + / Ca2 + exchange current of myocardial cells using rat ventricular myocytes isolated from collagenase-B. The results showed that the Ni2 + sensitive currents increased in a concentration-dependent manner with 10 μmol, 20 μmol and 40 μmol of dimethyl-amiloride, and increased by (27 ± 7)% at membrane potential of +5 0 mV (110 ± 5 2)%, (2 2 ± 1 ± 88)%, and (2 81 ± 80)% respectively when the membrane potential was -100 mV, (12 1 ± 4 3)% and %. There was no effect of taurine on the inward and outward currents of Na + / Ca2 + exchange