,Overexpression of PTEN Induces Cell Growth Arrest and Apoptosis in Human Breast Cancer ZR-75-1 Cell

• 作 者: Xiangyong LI Guanping LIN Binhua WU Xin ZHOU Keyuan ZHOU
• 机 构: Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China“
• 出 处: 生物化学与生物物理学报(英文版)
• 发表日期: 2007年10期
• 关键词: PTEN tumor suppressor gene breast cancer cell growth apoptosis

Phosphatase and tensin homolog (PTEN) is a tumor suppressor gene located at human chromosome 10q23, might play an important role in cell proliferation, cell cycle and apoptosis of cancer cells. In this study, the eukaryotic expression vectors pBP-wt-PTEN (containing a wild-type PTEN gene)and pBP-G129R-PTEN (containing a mutant PTEN gene) were used to transfect breast cancer ZR-75-1 cells. After transfection, ZR-75-1 cells expressing PTEN were obtained and tested. The blue exclusion assay showed the growth rate of the cells transfected with pBP-wt-PTEN was significantly lower than that of the control cells transfected with pBP-G129R-PTEN. Analysis of the cell cycle by flow cytometry showed that the progression from the G1 to the S phase was arrested in cells expressing wild-type PTEN. Some typical morphological changes of apoptosis were also observed in cells transfected with pBP-wt-PTEN, but not in those transfected with pBP-G129R-PTEN. This study shows that overexpression of PTEN in ZR-75-1 cells leads to cell growth arrest and apoptosis.