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用MTT比色、AgNORs银集结合图像分析以及抗Ⅲ型胶原免疫组化技术观察并证实了γ-IFN和地塞米松（Dexa）对成纤维细胞生长的抑制作用。结果如下：（1）培养第3d，250×103U／L的γ-IFN和100mg／L的Dexa对成纤维细胞生长的抑制率分别为43％和33％；（2）照后3、5d两种抑制剂对正常、受照射以及5-HT刺激的受照成纤维细胞增殖（OD值示之）均显示出明显的抑制作用；（3）250×103U／L浓度的γ-IFN和150mg／L的Dexa对成纤维细胞AgNORs合成显示最强的抑制作用；其AgNORs颗粒数在5-HT组分别为对照值的58％和56％，而AgNORs3个定量参数的均值分别为对照值的51％和44％2（4）上述浓度的两种抑制剂对5-HT刺激的成纤维细胞Ⅲ型胶原合成也显示明显的抑制作用，在培养第3dⅢ型胶原阳性细胞数分别为对照值的62％和43％。
The inhibitory effects of γ-IFN and Dexa on the growth of fibroblasts were observed and confirmed by MTT colorimetric analysis, AgNORs Ag-silver combined image analysis and anti-type Ⅲ collagen immunohistochemistry. The results were as follows: (1) The inhibition rate of fibroblast growth was 43% and 33% respectively at the 3rd, 250 × 103U / L γ-IFN and 100mg / L Dexa; (2) (3) 250 × 103U / L concentration of γ-IFN and 150mg / L of IFN-γ could effectively inhibit the proliferation of irradiated fibroblasts and irradiated 5-HT- L Dexa showed the strongest inhibition on AgNORs fibroblasts synthesis; the number of AgNORs particles in the 5-HT group were 58% and 56% of the control value respectively, while the mean value of AgNORs 3 quantitative parameters were 51% And 44% 2 (4) The two concentrations of these two inhibitors on 5-HT-induced fibroblast type Ⅲ collagen synthesis also showed significant inhibition in the culture of collagen type Ⅲ Ⅲ collagen positive cells were 62% And 43%.