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目的 探讨ｐ２１ 基因表达对神经母细胞瘤（ＮＢ） 发生发展的影响。方法 应用酶标链霉亲合素生物素（ＬＳＡＢ）免疫组化技术对２０ 例ＮＢ检测。结果 １８ 例（９０ ％） 可见ｐ２１ 表达阳性细胞，表达指数（ＥＩ）０ ％ ～４５．６ ％ 。有丝分裂核碎裂指数（ＭＫＩ）、瘤体直径与ＥＩ呈负相关，存活时间与ＥＩ呈正相关（ｒ 分别为－０ ．８９ 、－ ０．４９、０ ．４７）。ＥＩ＞２０％ 者ＭＫＩ和瘤体直径均显著低于或小于ＥＩ＜ ２０％ 者（ Ｐ＜ ０．０１），ＥＩ＞ ２０ ％ 者存活时间显著长于ＥＩ＜ ２０ ％ 者（Ｐ＜ ０．０５） 。结论 ｐ２１ 表达水平与之能否抑制ＮＢ细胞周期演进关系密切，表达水平高时，ＮＢ 细胞增殖活性受抑，ＭＫＩ低，预后好；反之，细胞周期演进快，ＭＫＩ高，预后差。
Objective To investigate the effect of p21 gene expression on the development of neuroblastoma (NB). Methods 20 cases of NB were detected by streptavidin-biotin (LSAB) immunohistochemistry. Results 18 cases (90%) showed p21 positive cells, expression index (EI) 0% ~ 45.6%. Mitotic nuclear fragmentation index (MKI), tumor diameter and EI was negatively correlated, survival time and EI were positively correlated (r were -0 .89, -0.49, 0.47). EI> 20%, MKI and tumor diameter were significantly lower or less than EI <20% (P <0.01), EI> 20% survival time was significantly longer than EI <20% . Conclusion The expression level of p21 is closely related to whether it can inhibit the cell cycle progression of NB cells. When the expression level is high, the proliferation activity of NB cells is inhibited and the MKI is low with a good prognosis. On the contrary, the cell cycle progression is rapid and MKI is high with poor prognosis.