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为探讨微量元素与自由基代谢的关系,本实验采用四氯化碳诱导肝损伤的大鼠模型,研究了中毒大鼠体内自由基和微量元素含量的动态变化。结果大鼠经四氯化碳致毒后,肝组织自由基浓度增加,肝脏中Cu,Zn-SOD含量增加,存在着自由基底物对超氧化物歧化酶的诱导合成作用。肝脏出现明显的脂质过氧化增强现象,MDA浓度增加,GSH降低和GSSG升高。实验还观察到脑组织亦发生轻度脂质过氧化增强。在上述变化的同时,与自由基代谢有关的微量元素Zn、Cu、Fe、Mn和Se也发生了明显的改变,其中肝脏中Zn、Cu和Se含量显著降低,而肝Fe却明显增高,在脑组织中表现为Zn的降低和Se升高。上述结果说明微量元素改变在肝损伤时的自由基代谢中具有一定的作用。
In order to explore the relationship between trace elements and free radical metabolism, we used a carbon tetrachloride-induced rat model of liver injury to study the dynamic changes of free radicals and trace elements in poisoned rats. Results After carbon tetrachloride poisoning, the concentration of free radicals in liver tissue increased and the contents of Cu and Zn-SOD in the liver increased. There was an induction effect of free radical on superoxide dismutase. Significant increase in liver lipid peroxidation, increased MDA concentration, decreased GSH and elevated GSSG. Experiments also observed that mild lipid peroxidation also occurred in brain tissue. At the same time, the trace elements Zn, Cu, Fe, Mn and Se related to free radical metabolism also changed obviously. The content of Zn, Cu and Se in the liver decreased significantly while the content of Fe in liver increased obviously. Brain tissue showed decreased Zn and increased Se. The above results indicate that changes in trace elements play a role in free radical metabolism during liver injury.