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目的 :本文报道为HCMV感染的一对朝鲜族夫妇头胎妊娠 18周 ,B超发现胎儿软组织损伤畸形 ,终止妊娠。对HCMV感染导致机体内遗传物质损伤发生机制进行初步探讨。方法 :HCMV DNAPCR检测 :常规外周血淋巴细胞培养染色体标本制备 ,G显带 ;SCE、微核标本制备。结果 :①妻子、丈夫、胎儿HCMV DNA均为阳性 ;②核型分析 :妻子为 46 ,XX、丈夫为 46,XY、胎儿为 46,XX ,与表型相一致 ;③SCE率 :同上顺次为 9.65 %、5 .95 %、9.2 5 % ,;④微核率 :同上顺序为 19‰、7 5‰、5‰。③④项均高于本实验室的正常人对照组平均值 ,即SCE率为 6 2 9%± 0 .2 9、微核率为 3 5 4‰± 1 71。结论 :HCMV感染与SCE率、微核率增高有直接关系。HCMV可能有直接损伤DNA或染色体的作用。
OBJECTIVE: In this study, a pair of Korean couples with HCMV infection were pregnant at 18 weeks of gestation. B-ultrasound revealed fetal soft tissue injury and deformity and terminated pregnancy. The mechanism of HCMV infection leading to damage of genetic material in the body is discussed. Methods: HCMV DNAPCR detection: routine peripheral blood lymphocyte culture chromosome preparation, G band; SCE, micronucleus preparation. Results: (1) HCMV DNA was positive in wife, husband and fetus; (2) karyotype analysis: wife 46, XX, husband 46, XY, fetus 46, XX, consistent with phenotype; ③SCE rate: 9.65%, 5.95%, 9.25%, respectively; ④Micronucleus rate: The same order as 19 ‰, 75%, 5 ‰. ③ ④ items were higher than the average of the normal control group in our laboratory, that is, the SCE rate was 62.9% ± 0.29, and the micronucleus rate was 345% ± 1. Conclusion: HCMV infection is directly related to SCE rate and micronucleus rate. HCMV may have a direct DNA or chromosomal damage.