舒尼替尼抑制小鼠骨髓来源树突状细胞表面PD-L1和PD-L2的表达

• 作 者: 赵飞龙 麦海星 董金凯 李学超 汤永永 陈立军 张斌
• 机 构: 安徽医科大学解放军307医院临床学院泌尿外科,军事医学科学院附属医院泌尿外科,军事医学科学院附属医院造血干细胞移植科,
• 出 处: 细胞与分子免疫学杂志
• 发表日期: 2015年01期
• 关键词: PD-L1 PD-L2 程序性死亡 舒尼替尼 树突状细胞 配体 肾细胞癌 小鼠骨髓 小鼠骨髓细胞 转移性肾癌

目的观察小鼠髓源性树突状细胞(DC)在舒尼替尼刺激下,其表面程序性死亡分子1配体1(PD-L1)和PD-L2的表达变化。方法取小鼠骨髓细胞,对照组加入二甲基亚砜,实验组分别加入(100、200、300)ng/m L舒尼替尼,刺激48 h,用流式细胞术检测DC表面PD-L1和PD-L2的表达水平。结果与对照组相比,实验组中成熟DC(m DC)和总DC(包括m DC和im DC)表面PD-L1的表达水平显著降低;表达PD-L1的未成熟DC(im DC)、m DC和DC百分比均显著降低,表达PD-L2的m DC百分比显著降低;表达PD-L2的DC百分比在100 ng/m L、300 ng/m L舒尼替尼组中显著降低。结论舒尼替尼可显著降低小鼠DC表面PD-L1、PD-L2的表达。

Objective To observe the changes of the expression of PD-L1 and PD-L2 on mouse dendritic cells (DCs) stimulated with sunitinib. Methods Bone marrow cells were obtained from mice and DMSO was added to the control group. Sunitinib (100, 200, 300) ng / m L was added to the experimental group for 48 h. Flow cytometry was used to detect PD- L1 and PD-L2 expression levels. Results Compared with the control group, the expression of PD-L1 on the surface of mature DCs (m DCs) and total DCs (including m DCs and im DCs) in the experimental group was significantly decreased; immature DCs (im DCs) expressing PD- The percentages of DC and DC were significantly decreased, and the percentage of m DC expressing PD-L2 was significantly decreased. The percentage of DCs expressing PD-L2 was significantly reduced at 100 ng / mL and 300 ng / mL sunitinib. Conclusion Sunitinib can significantly reduce the expression of PD-L1 and PD-L2 on the DC surface of mice.