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肝原发性类癌为一少见肿瘤,国内外报告甚少,其预后好于肝细胞癌,但仅凭光镜观察较难识别,作者对2例肝原发性类癌进行超微结构观察和免疫胶体金标记,现报道如下。材料与方法作电镜观察的组织待手术切除后立即用4％冷戊二醛固定,梯度酒精脱水,Epon-812包埋,LKB-V型切片机切出薄片,H-300型透射电镜观察。作免疫电镜的葡萄球菌A蛋白胶体金(SPAG)由本室应用鞣酸——柠檬酸钠还原法生产,直径10nm,在Sephacry1 S-400层析柱上分离纯化,抗NSE抗体由Dako公司生产(1:100稀释)。
Primary carcinoid in the liver is a rare tumor. There are few reports at home and abroad. The prognosis is better than that of hepatocellular carcinoma. However, it is difficult to identify only by light microscopy. The authors performed ultrastructural observation of primary carcinoid in 2 cases. And immunogold labeling is now reported below. Materials and Methods Electron microscopic observations were performed immediately after surgical resection with 4% cold glutaraldehyde, gradient alcohol dehydration, Epon-812 embedding, LKB-V microtome slicer, and H-300 transmission electron microscope observation. The staphylococcal protein A colloidal gold (SPAG) used for immunoelectron microscopy was produced in our laboratory using citric acid-sodium citrate reduction method, with a diameter of 10 nm, isolated and purified on a Sephacry1 S-400 column, and an anti-NSE antibody produced by Dako ( 1:100 dilution).