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目的研究半乳凝素3(Gal-3)在胰腺癌组织中的表达及其临床意义。方法收集40例患者胰腺癌组织及其配对的癌旁组织,采用免疫组化、Western blot法和RT-PCR检测Gal-3蛋白和mRNA表达,并分析其与临床病理参数间的关系。结果胰腺癌组织中Gal-3蛋白阳性表达率和mRNA表达量均高于癌旁组织(92.5%vs.12.5%和77.52±0.58vs.12.28±0.67)(P<0.01)。有淋巴结转移和病理分期Ⅱ期胰腺癌组织中Gal-3的阳性表达率高于无淋巴结转移和病理分期Ⅰ期(P<0.05),且Gal-3的蛋白表达与患者性别、年龄、肿瘤部位、肿瘤大小、分化程度和血管侵犯无显著相关(P>0.05)。结论 Gal-3在胰腺癌组织中过表达,且与病理分期和淋巴结转移密切相关。
Objective To study the expression of galectin-3 (Gal-3) in pancreatic cancer and its clinical significance. Methods Forty pancreatic cancer tissues and matched paracancerous tissues were collected. The expression of Gal-3 protein and mRNA was detected by immunohistochemistry, Western blot and RT-PCR. The relationship between the expression of Gal-3 and clinicopathological parameters was analyzed. Results The positive rates of Gal-3 protein and mRNA expression in pancreatic cancer tissues were higher than those in adjacent non-cancerous tissues (92.5% vs. 12.5% and 77.52 ± 0.58 vs. 12.28 ± 0.67, P <0.01). The positive expression rate of Gal-3 in stage Ⅱ pancreatic cancer with lymph node metastasis and pathological stage was higher than that in stage Ⅰ without lymph node metastasis and pathological stage (P <0.05), and the protein expression of Gal-3 was positively correlated with the gender, age, tumor location There was no significant correlation between tumor size, differentiation and vascular invasion (P> 0.05). Conclusion Gal-3 is overexpressed in pancreatic cancer and closely related to pathological stage and lymph node metastasis.