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目的通过研究EB病毒(EBV)基因在儿童系统性红斑狼疮(SLE)中的表达,探讨EBV在儿童SLE发病中的作用。方法 (1)分离20例SLE患儿和12例健康对照外周血单核细胞(PBMCs)。(2)用ELISA检测EBV-Ig G/Ig M阳性的SLE患儿,取其分泌EBV病毒的细胞上清液与提取的PBMCs共同培养12 d,提取培养后的PBMCs的RNA,用逆转录实时定量PCR(RT-PCR)的方法检测EBV基因的表达。结果 SLE患儿组伏期基因LMP1、LMP2、EBNA-1和裂解期基因BCRF1、BLLF1的表达均显著高于健康对照组,差异有统计学意义(分别为1.011±0.145比0.383±0.180,t=10.680,P<0.05;1.001±0.096比0.208±0.086,t=25.109,P<0.05;1.012±0.168比0.188±0.157,t=14.591,P<0.05;1.015±0.171比0.344±0.169,t=10.680,P<0.05;1.038±0.271比0.117±0.085,t=10.680,P<0.05)。结论儿童SLE患者中存在EBV基因的异常表达,EBV基因可能参与了SLE的发病过程。
Objective To investigate the role of Epstein-Barr virus (EBV) in the pathogenesis of childhood SLE by studying the expression of Epstein-Barr virus (EBV) gene in children with systemic lupus erythematosus (SLE). Methods (1) Twenty children with SLE and 12 healthy control peripheral blood mononuclear cells (PBMCs) were isolated. (2) EBV-Ig G / Ig M-positive children with SLE were detected by ELISA. The supernatant of EBV-secreting cells was co-cultured with the extracted PBMCs for 12 days. The RNA of cultured PBMCs was extracted, Quantitative PCR (RT-PCR) method to detect EBV gene expression. Results The expression of LMP1, LMP2, EBNA-1 and BCRF1 and BLLF1 in SLE children were significantly higher than those in healthy controls (1.011 ± 0.145 vs 0.383 ± 0.180, t = P <0.05; 1.015 ± 0.171 vs. 0.344 ± 0.169, t = 10.680, P <0.05; P <0.05; 1.038 ± 0.271 vs. 0.117 ± 0.085, t = 10.680, P <0.05). Conclusions The abnormal expression of EBV gene exists in children with SLE. The EBV gene may be involved in the pathogenesis of SLE.