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目的 研究糖尿病大鼠早期肾病的中西医造模方法。方法 利用单侧肾切除合并尾静脉注射链脲佐菌素 (STZ)及高脂饮食的方法造成糖尿病早期肾病 (DN)模型及痰浊血瘀中医证候模型。应用自拟的具有祛痰化瘀功效的中药糖克煎剂于造模成功后灌胃治疗 4周 ,以反证此中医证候模型是否成立。实验前后测定大鼠血糖、尿微量白蛋白 (Alb) ,血脂、血流变、肾小球滤过率 (用内生肌酐清除率表示 )。结果 尿微量白蛋白在造模一周后即有明显排出 ,肾小球滤过率、血脂及血流变也有改变。结论 利用单侧肾切除合并尾静脉注射链脲佐菌素及高脂饮食的方法 ,可以造成痰浊血瘀型糖尿病大鼠早期肾病动物模型
Objective To study the methods of making traditional Chinese medicine and western medicine for early nephropathy in diabetic rats. Methods The model of early diabetic nephropathy (DN) and TCM syndrome of phlegm and blood stasis were induced by unilateral nephrectomy combined with STZ and high fat diet. Apply the self-made traditional Chinese medicine with the expectorant and stasis effect of decoction in the model after successful gavage for 4 weeks to verify whether the TCM syndrome model is established. Before and after the experiment, blood glucose, urinary albumin (Alb), blood lipids, blood rheology, glomerular filtration rate (endogenous creatinine clearance rate) were measured. Results Urine microalbumin in the model a week after the obvious discharge, glomerular filtration rate, blood lipids and blood rheology have changed. Conclusion Unilateral nephrectomy with tail vein injection of streptozotocin and high-fat diet can cause phlegm and blood stasis type diabetic rats early nephropathy animal model