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以不同基因型大麦为材料，就麦芽计、MET及愈伤组织低温处理对大麦花粉离体培养的影响进行了研究。结果表明，在含有10％麦芽汁的诱导培养基上花药反应早，诱导率和绿茵率均有不同程度的提高；分化培养基中添加1．5mg／LMET可明显增强幼苗素质；在本试验条件下，以MET0.5～1.0mg／L＋IBA0．3mg／L配合使用（壮苗生极培养），有利于植株地上部与地下部的协调生长；愈伤组织经低温（5～8℃）保存效果良好，为大麦试管苗越夏提供了一条新途径。
Different genotypes of barley were used as materials to study the effects of malt, MET and callus on the pollen in vitro culture of barley. The results showed that anther reaction was early, the induction rate and the gentian rate increased on the induction medium containing 10% wort to varying degrees. Adding 1.5mg / LMET to the differentiation medium could obviously improve the seedling quality. Under the conditions of this experiment , With the combination of MET 0.5 ~ 1.0mg / L + IBA 0.3mg / L (strong seedling growth pole), it is conducive to the coordinated growth of shoots and underground parts of the plant. The callus was preserved at low temperature (5-8 ℃) Good, provides a new way for barley summer seedling test.