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本文在体外对新生大鼠（０～１ｄ）大脑皮层神经细胞进行原代培养的基础上，建立谷氨酸（Ｇｌｕ）对培养的皮层神经细胞兴奋毒损伤模型。通过检测乳酸脱氢酶释出率和形态学观察，证实了Ｇｌｕ对神经细胞存在兴奋毒作用。通过测定不同条件下Ｇｌｕ兴奋毒对培养的神经细胞造成损伤时细胞膜脂质过氧化物（ＬＰＯ）浓度，发现膜ＬＰＯ浓度与Ｇｌｕ剂量及作用时间紧密相关，表明Ｇｌｕ对神经细胞兴奋毒作用有自由基产生并介导了神经细胞的损伤。
Based on the primary culture of neurons in the cerebral cortex of newborn rats (0 ~ 1d) in vitro, a model of excitotoxicity of glutamate (Glu) to cultured cortical neurons was established. Through the detection of lactate dehydrogenase release rate and morphological observation, confirmed the Glu excitotoxic effect on nerve cells. By measuring the concentration of LPO in cultured neurons caused by Glu excitotoxicity under different conditions, it was found that the concentration of LPO was closely related to the dosage of Glu and the action time, which indicated that Glu had the excitatory effect on nerve cells Bone produces and mediates nerve cell damage.