论文部分内容阅读
目的从分子水平鉴定丙型肝炎病毒（ＨＣＶ）宫内感染的可能性，为确定ＨＣＶ垂直传播提供更直接的依据。方法分别用免疫分析法和逆转录－聚合酶链反应检测婴儿和母亲的血清抗ＨＣＶ和ＨＣＶＲＮＡ，对母婴ＨＣＶＲＮＡ阳性的血清作ＨＣＶ包膜糖蛋白Ｅ２高可变区（Ｅ２ＨＶ）序列分析和比较。结果１１例ＨＣＶ感染的母亲所生的婴儿有６例生后脐血和静脉血抗ＨＣＶ阳性，５例于１～５个月阴转，另１例持续阳性达１３个月；５例于生后ＨＣＶＲＮＡ阳性，３例于１～６个月自然转阴，２例持续阳性分别达９个月和１３个月；对２例持续ＨＣＶＲＮＡ阳性的婴儿和其母亲Ｅ２ＨＶＲ序列分析发现，生后母婴间的氨基酸同源性高达９３．３３％，而２例母亲间同源性６２．２２％。结论丙型肝炎可以发生母婴传播，并可能为宫内感染。
Objective To identify the possibility of intrauterine infection of Hepatitis C virus (HCV) at the molecular level and provide a more direct basis for determining the vertical transmission of HCV. Methods Serum anti-HCV and HCVRNA were detected by immunoassay and reverse transcription-polymerase chain reaction (RT-PCR), and HCV E2 glycoprotein (E2V) sequences were analyzed and compared between HCVRNA positive sera and maternal serum . Results Six of the infants born to 11 HCV-infected mothers were positive for anti-HCV in their umbilical and venous blood after birth, 5 were negative in 1 to 5 months and the other 1 were persistent in 13 months. After 3 days, HCV RNA was positive, and 3 cases turned negative from 1 to 6 months. The positive rate of HCV RNA was 9 months and 13 months, respectively. The sequence analysis of E2HVR in 2 consecutive HCVRNA positive infants and their mothers found that after birth, The amino acid homology was 93.33%, while the homology between two mothers was 62.22%. Conclusion Hepatitis C can occur in mother-to-infant transmission and may be intrauterine infection.