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目的 探讨弓形体在C57BL/6(B6)小鼠眼内诱发的细胞凋亡。方法 凋亡的检测是通过末端脱氧核苷转移酶介导的脱氧尿核苷三磷酸生物素缺口末端标记 (TUNEL)技术 ,我们同时对眼内接种 50或 50 0个速殖子后不同时间点的病理变化进行了分析。结果 在接种 50个速殖子的小鼠眼内 ,接种后 1到 2天 ,可见到前房内少量炎症细胞和角膜细胞的凋亡 ,但接种后第 4天则未检测到细胞凋亡。而在注射了 50 0个寄生虫的小鼠眼内 ,前房和玻璃体内均可见到显著增多的炎症细胞凋亡 ,第 1天即可见到前房内炎症细胞和角膜内基质细胞的凋亡 ,且凋亡的基质角膜细胞在第 4天明显地增多。第 2天在玻璃体出现一定数量的凋亡炎症细胞。第 4天沿视网膜内界膜及神经纤维层可见少量凋亡视网膜细胞。结论 以上结果提示 ,弓形体感染的眼内浸润炎症细胞的凋亡可能是杀灭这种寄生虫的机制之一。但是大量的炎症细胞凋亡在眼弓形体病中也可能造成对眼组织的损害。
Objective To investigate the apoptosis of Toxoplasma gondii in C57BL / 6 (B6) mice. Methods Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated deoxycytidine triphosphate triphosphate nick end labeling (TUNEL) technique. We also tested for 50 or 50 tachyzoites at different time points after intraocular inoculation The pathological changes were analyzed. Results Apoptosis of a small number of inflammatory cells and corneal cells in the anterior chamber was observed in the eyes of 50 tachyzoites inoculated 1 to 2 days after inoculation, but no apoptosis was detected on the 4th day after inoculation. In the mice injected with 50 parasite, there was a marked increase in apoptotic inflammatory cells in the anterior chamber and in the vitreous. The apoptosis of inflammatory cells in the anterior chamber and stromal cells in the cornea was observed on the first day , And apoptotic stromal keratocytes significantly increased on the 4th day. On the second day a certain number of apoptotic inflammatory cells appeared in the vitreous. On the fourth day, a small number of apoptotic retinal cells were seen along the inner limiting membrane and the nerve fiber layer of the retina. Conclusion The above results suggest that apoptosis of Toxoplasma gondii-infiltrating inflammatory cells may be one of the mechanisms to kill this parasite. However, a large number of inflammatory cell apoptosis in ocular Toxoplasmosis may also cause damage to the eye tissue.