论文部分内容阅读
利用Nafion(全氟聚苯乙烯磺酸溶液)-氧化石墨烯复合物、硫堇和纳米金构建了H2O2酶传感器。首先将氧化石墨烯分散在体积分数0.2%Nafion溶液中制得Nafion-氧化石墨烯的复合物,并将其固定在玻碳电极表面,通过静电吸附将带正电荷的硫堇吸附到Nafion-氧化石墨烯复合膜修饰的玻碳电极表面,再利用静电吸附将纳米金修饰于电极上,通过纳米金来固定辣根过氧化物酶从而制得H2O2传感器。用循环伏安法和计时电流法考察该修饰电极的电化学特性。H2O2浓度为5.5×10-6~1.0×10-3mol/L时,酶电极的响应电流值与H2O2的浓度呈良好的线性关系,检出限为1.80×10-6mol/L。
The H2O2 enzyme sensor was constructed using Nafion (perfluoropolystyrene sulfonic acid solution) -granl oxide composite, thionine and gold nanoparticles. First, graphene oxide was dispersed in a volume fraction of 0.2% Nafion solution to prepare a composite of Nafion-graphene oxide and immobilized on the surface of the glassy carbon electrode. The positively charged thionine was adsorbed to the Nafion-oxidation Graphene composite film modified glassy carbon electrode surface, and then the use of electrostatic adsorption of gold nanoparticles modified on the electrode, through the gold nanoparticles to immobilize horseradish peroxidase thus prepared H2O2 sensor. The electrochemical properties of the modified electrode were investigated by cyclic voltammetry and chronoamperometry. When the concentration of H2O2 was 5.5 × 10-6 ~ 1.0 × 10-3mol / L, the response current of the enzyme electrode showed a good linear relationship with the concentration of H2O2, and the detection limit was 1.80 × 10-6mol / L.