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用免疫细胞化学方法研究了大鼠肾毒血清肾炎（nephrotoxicnephritis,NTN）发病头7天肾组织中血小板衍生的生长因子（platelet－derivedgrowthfactor，PDGF）增生细胞核抗原（proliferatingcellmuclearantigen,PCNA），和单核／巨噬细胞的变化以及检测这些细胞之间及其与肾功能之间的相关性。结果表明，NTN大鼠发病第1天，肾小球内未能检出PDGF.发病第3天，肾小球内出现PDGF，分布在血管间质区，与浸润的单核／巨噬细胞的分布一致，两者细胞数呈明显正相关。发病第7天，PDGF染色阳性细胞数与PCNA，蛋白尿和血肌酐亦呈显著正相关。提示NTN时，PDGF并不参与肾小球最初的免疫损伤机制，而可能为损伤后浸润的单核细胞和受刺激的血管间质细胞所释放，并在肾炎发展过程中起作用。
The proliferative cell nuclear antigen (PCNA) of platelet-derived growth factor (PDGF) in renal tissue in the first seven days after the onset of nephrotoxicnephritis (NTN) in rats was studied by immunocytochemistry. Changes in macrophages, and the correlation between these cells and their renal function. The results showed that PDGF could not be detected in the glomerulus on the first day after the onset of NTN.During the third day after the onset of PDGF, PDGF appeared in the glomerulus and distributed in the interstitial region of the blood vessels, which was associated with infiltration of monocytes / macrophages The same distribution, the two cell number was positively correlated. On the 7th day after onset, there was a significant positive correlation between the number of PDGF-positive cells and PCNA, proteinuria and serum creatinine. When NTN was suggested, PDGF did not participate in the initial mechanism of glomerular immunosuppression, but may be released from infiltrating monocytes and stimulated vascular stromal cells after injury and play a role in the development of glomerulonephritis.