Determination of amino acids in the traditional Chinese medicine formula Sheng-Mai-San by high perfo

• 作 者: Na Xu Boyang Yu Yongqing Yan Danni Zhu
• 机 构: DepartmentofComplexPrescriptionofTCM,,ChinaPharmaceuticalUniversity,NO.639,LongmianRoad,Nanjing21119
• 出 处: 第四届华东地区色谱、质谱学术报告会
• 发表日期: 2010年11期
• 关键词: Sheng-Mai-San Free amino acids analysis High performance capillary electrophores

　　A method was developed for the quantitative analysis of amino acids in Sheng-Mai-San (SMS), using micellar electrokinetic chromatography (MEKC) in uncoated fused silica capillaries and UV detection.The influence of different parameters, such as the organic modifier and applied voltage on the MEKC separation of amino acids was investigated.A good compromise between resolution and migration time was obtained by use ofa 70mM SDS on borate buffer (pH 9.5, I=40mM) with 15% acetonitrile.That method was applied to determine amino acid in SMS and SMS market formulas samples.