论文部分内容阅读
The growth and development of solid tumors is critically dependent on angiogcnesis in the absence of which tumors remain dormant and unable to metastasize.It is established that administration of angiogenesis inhibitor (e.g.endostatin or angiostatin) results in the inhibition ofangiogenesis and tumor development in animal models.The primary objective of this study is to explore the feasibility of inhibiting tumor growth via the delivery of endostatinangiostatin (Endo::Angio) fusion gene by baculovirus, which is an insect virus and emerges as a new gene therapy vector in recent years.We first constructed two recombinant baculoviruses: Bac-hEA harboring the Endo::Angio fusion gene under the transcriptional control of eytomegalovirus immediate-early promoter, and Bae-ITR-hEA harboring the same transgene cassette flanked by the adeno-assoeiate virus (AAV) inverted terminal repeats (ITRs).