Identification of miRNome in development and differentiation of dendritic cells by massively paralle

• 作 者: SuXiaoping;QianChen;HouJin;ZhangQian;CaoXuetao;
• 机 构: Institute of Immunology and National Key Laboratory of Medical Immunology, Second Military Medical U
• 出 处: 全军免疫学第七届学术交流会暨免疫学新进展主题论坛
• 发表日期: 2009年期

　　Aim & Significance:MicroRNAs (miRNAs) are differentially expressed spatially and temporally in many type of immune cells which suggest that miRNAs have potentially widespread functions in the regulation of development and differentiation of immune cells.In an effort to gain further insight into the role of miRNAs in development and differentiation of immune cells.Method:we have applied the Illumina massively parallel sequencing to carry out an in-depth analysis of the miRNome at different stages of dendritic cells (DCs) (bone marrow (BM) cells,immature DCs (imDCs),mature DCs (maDCs),differentiated DCs(diffDCs)).Results:We identified 400 miRNAs and 35 miRNA clusters differentially expressed in the processes of DCs differentiation and development.