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Background: Inferior alveolar nerve block(IANB)of the mandible is commonly used in the oral cavity.These anesthetic techniques are used for dental procedures by dentist.This study had to evaluate the success rate of the first inferior alveolar nerve block administered by dental practitioners.Methods:One hundred and six inferior alveolar nerve block(INAB)procedures were carried out by volunteer dental practitioners of Mahidol University who had never performed an inferior alveolar nerve block before.The practitioners were divided into 12 groups with their advisors by randomized control trials.We recorded the success rate via pain VAS scores.Results:85.26% of the dental practitioners used the standard method to locate the anatomical landmarks and injected the local anesthetics at the correct position with the barrel of the syringe parallel to the occlusal plane of the mandibular teeth.Whereas,68.42% the dental practitioners injected the local anesthetics on the right side using left index finger for retraction.This study found that half of the dental practitioners yielded an onset time of approximate 0-5 mins(47.37% for subjective onset and 43.16% for objective onset),whilst the duration of the IANB was approximately 240-300 minutes(36.84%)after the initiation of numbness.Moreover,the VAS pain scores were 2.5±1.85 and 2.1±1.8 whilst injecting and delivering local anesthesia,respectively.Conclusions:The only recorded factor that affected the success of the local anesthetics was the administering practitioner.This reinforces the notion that local anesthesia is technique-sensitive procedure.