【摘 要】
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Objective: To investigate the differential expression of TUBB gene in myelodysplastic syndrome (MDS) patients with and without leukemic evolution and to investigate the effects of the down-regulation
【机 构】
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Department of Hematology,Huashan Hospital,Fudan University,Shanghai,China
【出 处】
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22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
论文部分内容阅读
Objective: To investigate the differential expression of TUBB gene in myelodysplastic syndrome (MDS) patients with and without leukemic evolution and to investigate the effects of the down-regulation of TUBB gene on SKM-1 cell line.Methods: Based on our nested case-control study cohort of MDS patients, we chose paired patients in 1:1 ratio according to age, gender, WHO subtype, IPSS cytogenetic subgroup, and follow-up period (≥1 year).We examined TUBB gene expression changes in paired patients from the case group (with evolution)and control group (without evolution) by using quantitative real-time PCR.We used small interfering RNA to down-regulate the TUBB gene expression in SKM-1 cell line.The effects of TUBB-siRNA on the growth rate, colony formation percentage,and ultrastructure of SKM-1 were tested using the CCk-8kit, flow cytometry, and a soft agar and transmission electron microscope, respectively.
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