【摘 要】
：
In this study,Candida albicans was selected to study the antifungal activity of composite medicinal plant extracts(CMPEs),which was composed of Carthamus tinctorius,Lithospermum erythrorhizon,Solanum
【机 构】
：
School of Medicine and Nursing,Chengdu University,Chengdu,610106;School of Life Science and Engineer
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
In this study,Candida albicans was selected to study the antifungal activity of composite medicinal plant extracts(CMPEs),which was composed of Carthamus tinctorius,Lithospermum erythrorhizon,Solanum indicum,Cymbopogon distans and other natural plant extracts and the solvent of ethanol.Minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of CMPEs against Candida albicans were determined by Oxford cup assay system.And the effects of CMPEs with different concentration on Candida albicans during different disposal time were studied by cell damage experiments,such as antimicrobial kinetics,fungal growth curve,alkaline phosphatase(AKP)activity,the permeability and integrity of cell membrane and ultra microstructure of fungi observation and so on.The results showed that the(MIC)and(MBC)of CMPEs against Candida albicans were 75%and 80%.With the extension of treated time,the growth of the Candida albicans was significantly inhibited and also the cytoderm permeability of the increased,made the thallus extracellular AKP to increase,the integrity of the cellular structure was undermined,and the electronic conductivity value of the fungal solution increased obviously,fungal electrolyte contents were leaked which will affect the environment in the cell and the stability of cell membrane.Candida albicans thallus began to shrink,out of shape and rough in the surface,distorted;the cytoderm was collapsed and broken,the cytoplasm leak out which lead to the deaths of the fungi ultimately.
其他文献
Homologous recombination(HR)is one of major mechanisms that repair DNA double strand breaks(DSBs)and helps maintain genome stability.Many genes involved in HR contribute to hereditary and spontaneous
Calcineurin B(CNB)is the regulatory subunit of Calcineurin(CN),which is the only known Ca2+/calmodulin-dependent serine/threonine protein phosphatase.We founded its anticancer function.We got CNB by t
NDRG2,a member of the N-myc downstream-regulated gene family,was first cloned in our laboratory and is regarded as a tumor suppressor gene that is transcriptionally repressed by c-Myc.It has been show
猪圆环病毒2 型(PCV2)是引起断奶仔猪多系统衰竭综合征的主要病原.PCV2-Cap 是病毒的主要免疫保护性抗原，是研制基因工程亚单位疫苗的理想靶抗原.口蹄疫病毒(FMDV)是感染偶蹄目动物的重要病原.FMDV 衣壳蛋白VP1为病毒的主要免疫原性蛋白，能够诱导机体产生中和抗体.研究表明，利用重组蛋白研制的亚单位疫苗对PCV2和FMDV 感染具有良好的免疫效果，因此研制同时预防这两种病毒性疫病的二
Asymmetric cell division(ACD)is an evolutionarily conserved division mode used by stem and progenitor cells to create two daughter cells with distinct fates.Typically,one daughter cell retains self-re
多胺(polyamines,PA)包括腐胺(putrescine,Put),亚精胺(spermidine,Spd)和精胺(spermine,Spm),是一类普遍存在于细胞内能促进细胞生长与分化的小分子化合物.研究表明,多胺含量与肿瘤细胞增殖及细胞恶变密切相关,因此,通过干扰正常的多胺代谢途径,改变肿瘤细胞中多胺的含量,可有效地发挥抗肿瘤效应.多胺的生物合成始于鸟氨酸的脱羧基反应,鸟氨酸在鸟氨酸脱羧
The maladjustment of pro-inflammatory and anti-inflammatory had been acknowledged as one of the chief causations of inflammatory diseases and even cancers.Previous studies showed that plant-derived bo
Purpose:The aim of the study was to investigate the expression of mitogen activated protein kinase p38(p38MAPK),nuclear transcription factor kappa B(NFκ B)and tumor necrosis factor-α(TNF-α)in rats wit
Molecular imaging is powerful tool to explore the distribution or the function of a given target,thus has drawn significant attention in the fields of chemical biology and disease diagnosis.CD22 is a
HBeAgases 是一组蚯蚓蛋白酶,具有降解纤连蛋白和乙型肝炎e 抗原的作用。免疫组织化学的结果显示,HBeAgases 由蚯蚓的上消化道产生,定位于环节之上的消化道系统内。为了研究HBeAgases 在肠道内的吸收,本文作者研究了这些蛋白酶在肠壁和血液内的分布。采用HBeAgases 喂食小鼠,通过EFE-Ⅲ-1 的抗体进行标记可以观察到,HBeAgases 分布在胃肠壁的囊泡内,蛋白酶的含量