【摘 要】
：
Calcineurin B(CNB)is the regulatory subunit of Calcineurin(CN),which is the only known Ca2+/calmodulin-dependent serine/threonine protein phosphatase.We founded its anticancer function.We got CNB by t
【机 构】
：
Department of Biochemistry and Molecular Biology.Beijing Normal University.Beijing Key Laboratory.Be
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
Calcineurin B(CNB)is the regulatory subunit of Calcineurin(CN),which is the only known Ca2+/calmodulin-dependent serine/threonine protein phosphatase.We founded its anticancer function.We got CNB by technique for genetic engineering more than from animal cells which may be an anticancer drug.We founded CNB had shown strong,selective antiproliferative activity against HepG2 cell lines.CNB could also induced apoptosis of HepG2 cell lines.We focused at studying the involvement of apoptotic pathway.CNB significantly inhibited proliferation by inducing caspasedependent apoptosis.CNB was found to trigger intrinsic apoptotic pathway as indicated by depolarization of the mitochondrial inner membrane,modulated expression of Bcl-2 and Bcl-xl.CNB also banded to BCL-xl,leading to loss of its association with Bax.CNB suppressed expression of Bax and Bim and increased release of cytochrome c.In vivo,CNB targeted the tumor of mice.And CNB effectively suppressed the proliferation of tumor in mice.These results suggest that anticancer mechanisms of action of CNB could involve apoptotic pathways,dysregulation of mitochondrial functions and CNB could be an effective anti-cancer drug.
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