Salic Acid Functionalized Quantum dots as Fluorescent Nanoprobe Target for CD22-positive Human Lymph

• 作 者: Meijiao Zhang Lele Ding Qi Zhou Zimiao Weng Guangbo Ge Dachang Wu Jie Hou
• 机 构: Department of Biotechnology,College of Basic Medical Sciences,Dalian Medical University,Dalian,11604
• 出 处: 中国生物化学与分子生物学会2016年全国学术会议
• 发表日期: 2016年3期
• 关键词: CD22 target Salic acid Quantum Dots(QDs)

　　Molecular imaging is an important topic which has draw significant attention in the fields of chemical biology and diagnosis of disease.CD22 is a 140kDa transmembrane sialo-adhesion protein highly expressed on mature B cells and B cells lymphomas,which is also an important target for lymphomas treatment.Its been proposed that targeting CD22 presents pharmaceutical interest in autoimmune diseases,such as systemic lupus erythematosus and non-Hodgkin lymphoma.Herein,a novel salic acid functionalized quantum dots was synthesized and used for detection of cancer cells overexpressing the CD22.N-Acetylneuraminic acid(Neu5Ac),as the recognition part of the nanoprobe,was coupled to amino groups CdSe/ZnS quantum dots(QDs)surface to prepare SA-QDs through acetylation reaction with EDC as cross linker.The obtained nanocrystals with a photoluminescence quantum yield of 70%were purified via ultrafiltration and high quality nanoparticles were obtained.The average size of SA-QD nanoparticle was about 25nm,and its fluorescent emission wavelength was 560nm.The low cytotoxicity of both bare and SA-functionalized QDs was verified through CCK8 assays.The ability of SAQDs targeting to cancerous cells was investigated by comparing the uptake of nanoparticles by CD22+cells(Daudi)and CD22-cells(HepG2)by CLSM.Specificity of the SA-QDs fluorescent nanoprobes targeting towards CD22 was verified with cellular uptake inhibition assay,in which Daudi cells were incubated with both SA-QDs and free salic acid.In addition,the specificity was also confirmed by the colloation of the immunofluorescence staining of anti-CD22 and the cellular uptake of SA-QDs.All these findings suggested that this practical fluorescent nanoprobe strategy can potentially facilite the CD22 molecular imaging in biology and diagnosis of disease.