【摘 要】
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Aestivation is an adaptation of the sea cucumber Apostichopus japonicus to high temperature.To date,however,little is known about the intrinsic mechanisms of A.japonicus to cope with the rising temper
【机 构】
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School of Marine Sciences,Ningbo University,Ningbo,315211
【出 处】
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“全球变化下的海洋与湖沼生态安全”学术交流会
论文部分内容阅读
Aestivation is an adaptation of the sea cucumber Apostichopus japonicus to high temperature.To date,however,little is known about the intrinsic mechanisms of A.japonicus to cope with the rising temperature in aquaculture at the metabolic level.In the present study,the global metabolomic responses were characterized in the muscles of A.japonicus at two threshold thermal points,20 and 25 ℃,by 1H NMR-based metabolomics.The results showed that both temperatures disturbed energy metabolism and induced osmotic regulation based on the metabolic biomarkers such as elevated threonine,alanine,arginine,glutamate,tyrosine,histidine,glycine,fumarate,glucose,AMP and decreased ATP,aspartate,hypotaurine,choline after exposure for 0 and 3 days.At 7 days thermal-stress,several metabolites were similarly altered including the increased glutamine,glycine,glucose,AMP,tyrosine,histidine and decreased ATP at 20 °C.Surprisingly,a large amount of amino acids(valine,leucine,isoleucine,threonine,arginine,glutamate,phenylalanine)markedly declined at 25 ℃.In addition,the mRNA expression levels of HSP70 and ATP synthase as well as SOD activity and MDA content were also investigated,and their expression levels were significantly altered at different exposure conditions.Our present results provided new insights into thermal-stressed effects on A.japonicus at metabolic responses.
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