【摘 要】
：
The HAP complex occurs in many eukaryotic organisms and is involved in multiple physiological processes.HAP are ubiquitous transcription factors consisting
【机 构】
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Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, Beijing Forestr
【出 处】
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2rd International Symposium on Integrative Plant Biology第二届国
论文部分内容阅读
The HAP complex occurs in many eukaryotic organisms and is involved in multiple physiological processes.HAP are ubiquitous transcription factors consisting of three distinct subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (also called NF-YB/CBF-A), and HAP5 (also called NF-YC or CBF-C).Here we found that in Picea wilsonii, HAP5 (PwHAP5), a putative CCAAT-binding transcription factor gene, is involved in pollen tube development and control of tube orientation.Quantitative real-time reverse transcription-polymerase chain reaction showed that PwHAP5 transcripts were expressed strongly in germinating pollen and could be induced by Ca2+.Overexpression of PwHAP5 in pollen altered pollen tube orientation, whereas the tube with PwHAP5RNAi showed normal growth without diminishing pollen tube growth.
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