【摘 要】
：
Aims: To investigate the effect of electric field on integrinβ1 signaling and the induction of epithelial-to-mesenchymal transition (EMT) in human lens epi
【出 处】
：
西湖国际白内障及人工晶状体高峰论坛
论文部分内容阅读
Aims: To investigate the effect of electric field on integrinβ1 signaling and the induction of epithelial-to-mesenchymal transition (EMT) in human lens epithelial cells (HLE-B).Methods: Cultured HLE-B cells were exposed to an EF at 100mV/mm for 6h, 12h and 24h.Cell morphology was observed under an inverted microscope.The expression of E-cadherin, Vimentin and integrinβ1was examined by immunofluorescence.The mRNA levels of E-cadherin, Vimentin and integrinβ1 expression were evaluated with quantitative real-time polymerase chain reaction (qRT-PCR).The protein levels of E-cadherin,Vimentin, integrinβ1 and phosphorylated focal adhesion kinase (pFAK) expression were examined with Western blot.Experiments were performed in the presence or absence of anti-integrinβ1 antibody and anti-FAK antibody.
其他文献
目的分析高度近视眼应用SRK/T公式与优化Haigis公式计算人工晶状体（IOL）度数对白内障术后屈光误差的分布情况及影响因素，探讨2种公式的精确度。方法回顾性研究。选取2011年7月至2015年3月来温州医科大学附属眼视光医院行手术治疗，眼轴长度（AL）≥26 mm的高度近视白内障患者38例（63眼）。术前用IOLMaster检查AL、角膜屈光力（K）、前房深度（AC），应用SRK/T公式及优化
目的：探讨在内质网应激发生下Salubrinal对人晶状体上皮细胞(HLEC)的保护作用及机制方法：应用H202诱导HLEC建立氧化应激模型,诱导内质网应激(ERS)的发生。加入不同浓度Salu
血管内皮生长因子(VEGF)是糖尿病性视网膜病变(DR)发病过程中的重要调控因子。我们前期研究发现糖尿病大鼠的视网膜中琥珀酸的含量明显增加,沉默琥珀酸的特异性受体--G蛋
目的：利用OQAS视觉质量分析系统比较高度近视患者行有晶体眼人工晶体(phakic intraocular lens,p-IOL)植入术后与准分子激光原位角膜磨镶术(LASIK)术后的视觉质量.方法：回
Objective: To investigate the effect of diabetic condition on the key oxidation repair enzymes of the thioltransferase (TTase) in human lens.Methods: The an
目的：使用OQAS生物测量技术,比较球面及非球面人工晶状体对年龄相关性白内障患者术后视觉质量的影响.方法：将72眼年龄相关性白内障手术患者随即分为球面晶状体植入组及非球
目的：评价白内障超声乳化手术对Fuch's角膜营养不良患者角膜的影响.方法：将同期同一手术医师行白内障手术的患者分为Fuch's角膜营养不良组(A组,4例),及单纯性白内障组(B组,