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It is generally accepted that perylene bisimide (PBI) derivatives are widely used for investigation of self-assembly processes and relevant mechanisms due to their special photo-electronic properties and strong π-π interaction.Based on the previous work of our laboratory,we specially designed and synthesized an asymmetric PBI derivative with cholesterol and pyrene as the modifying structures.It was demonstrated that energy transfer (ET) could take place between pyrene and PBI in solution state (Fig.1).