Single-Molecule Force Spectroscopy Study of RNA Disassembly During the Infection of Tobacco Mosaic V

来源 :4th International Supramolecular System Symposium(ISSS2015)( | 被引量 : 0次 | 上传用户：chongfengli

【摘要】

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To explore the disassembly mechanism of tobacco mosaic virus (TMV) during infection, we have used AFM-based single-molecule force spectroscopy[1,2] to mimic/fol

【作者】

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Ying Chen Ningning Liu Bo Peng Yuan Lin Qian Wang Zhaohui Su Wenke Zhang Jiacong Shen

【机构】

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StateKeyLaboratoryofSupramolecularStructureandMaterials,CollegeofChemistryJilinUniversity,Changchun,

【出处】

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4th International Supramolecular System Symposium(ISSS2015)(

【发表日期】

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2015年9期

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To explore the disassembly mechanism of tobacco mosaic virus (TMV) during infection, we have used AFM-based single-molecule force spectroscopy[1,2] to mimic/follow the process of RNA disassembly from protein coat of TMV by the replisome in vivo, under different pH and Ca2+ concentration, respectively.Dynamic force spectroscopy revealed the unbinding energy landscapes as that at pH 4.7 the disassembly process is dominated by one energy barrier,while at pH 7.0 the process is dominated by two energy barriers.

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Single-Molecule Force Spectroscopy Study of RNA Disassembly During the Infection of Tobacco Mosaic V

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