猪精子冷冻保存过程中的表观遗传变化研究

来源 :中国畜牧兽医学会动物繁殖学分会第十七届学术研讨会 | 被引量 : 0次 | 上传用户:1by1
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
引言/目的 在猪精液冷冻保存过程中,冷冻引起精子结构和功能的变化,影响精子的活力和受精能力,导致冷冻精液人工授精配种受胎率降低、窝产仔数减少.与新鲜精液人工授精相比,猪冷冻精液人工授精的使用率仅1%.尽管在冷冻稀释液成分及冷冻处理程序等方面做了大量研究,但冷冻损伤的分子机制仍不清楚.研究表明,冷冻能够造成精子DNA甲基化、组蛋白乙酰化等表观遗传学变化,但存在争议.冷冻是否引起猪精子表观遗传学的变化尚未见报道.因此,本试验探讨猪精子冷冻过程中精子的表观遗传变化,为进一步优化猪冷冻保存稀释液、改善猪冷冻精液的保存效果提供科学的依据.
其他文献
In order to solve the problems of short shelf life of Trichoderma harzianum T4 formulations, new formulations of microbial pesticide is prepared for industrial production and agricultural application.
The addition of species of Trichoderma to compost is a widespread technique used to control different plant diseases.The biological control activity of these species is mainly attributable to a combin
会议
Establishment of root symbiosis has been reported as one of the key drivers of biocontrol success for members of the fungal genus Trichoderma.The ability to colonise and then proliferate over time wit
Phenotypes that differentiate one individual from another arise by any one or a combination of genetic events (including DNA sequence changes such as base substitution, deletion, acquisition or chromo
The potato is the third most important food crop in the world.It is a fundamental element in the food security for millions of people across South America.In Brazil, potato is grown on approximately 1
Tirchoderma viride Tr9701 is a potential antagonist of plant pathogenic fungi and cellulose decomposing ability, possessing high utilization value of biological control.In this paper, the effect of li
会议
Trichokonins(TKS) is one of peptaibols which from Trichoderma pseudokoningii SMF2.It has good antibacterial activity on some plant pathogens.Ailanthus altissima is a kind of the important ancient tree
A dichlorvos degradation related gene, pon, which was investigated mostly in mammals, was obtained in Trichoderma atroviride (T23) by homology cloning in this study.Based on cDNA template of T23, pon-
Trichoderma atroviride SG3403 showed high biocontrol activity against southern corn leaf blight (SCLB) caused by Cochliobolus heterostrophus race O.Microscopic observations revealed that the strain co
引言 由于家畜人工授精技术的空前发展,促进了种公畜精液稀释保存液的不断地改进.稀释液中加入一定量的奶类和卵黄,具有调节渗透压、降低精液电解质浓度、保护精子膜等作用,有利于精子的存活.卵黄对精子保护作用的机理尚不清楚;另外,卵黄还可能携带着病原微生物影响精子的受精,增加疾病传播的风险.因此,寻找成分稳定无污染的替代物显得非常重要.本实验研究分别探讨了添加脱脂奶粉替代牛奶和大豆卵磷脂替代卵黄在精液低温
会议