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Trichoderma atroviride SG3403 showed high biocontrol activity against southern corn leaf blight (SCLB) caused by Cochliobolus heterostrophus race O.Microscopic observations revealed that the strain could cause pathogen mycelium death in the confrontation culture assay.Furthermore, spraying SG3403 inoculant at the concentration of 1×104 spores/ml over corn seedling leaf prior to inoculation of SCLB pathogen spore suspension was able to protect the corn from the pathogen infection by 69.86% and 51.62% under greenhouse and field conditions, respectively.