【摘 要】
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Building maize pan-genome is of major importance because there are more variable between maize individuals than human and apes combined.Here we integrated next generation sequencing data of 79 maize i
【机 构】
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Institute of Crop Science,National Key Facility of Crop Gene Resources and Genetic Improvement,Chine
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Building maize pan-genome is of major importance because there are more variable between maize individuals than human and apes combined.Here we integrated next generation sequencing data of 79 maize inbred lines within wide genetic background with each material 10 depth.Filtering error reads and reads from the repeat region,we identified 870Mb novel contigs from joint assembly.After performing repeat masking and filtration of contamination of bacterial,fungi,mitochondrial and chloroplast,we got 490Mb clean novel contigs.40 ％of contigs are line-specific,and 85 ％of contigs are population-specific.24 ％of them have evidence in individual assembly with identity ＞ 99％.At least 38 ％pan-transcrptome data can be mapped onto the clean novel contigs.Novel contigs location evidenced by individual assembly showed their distribution was constant with FGS enrichment.The extended contig was 271Mb and the average N50 of extended novel contig was 1.5Kb.
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