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Treatment of breast cancer requires multidisciplinary modalities: including surgery, chemo-, radio-, hormonal and targeted therapy.Chemotherapy can be administered before or after surgery to achieve the maximal benefit.However,a number of breast cancer patients develop chemoresistance.Mechanism of chemoresistant in breast cancer is complex and is not well understood.In this study, two-dimensional electrophoresis (2-DE) analysis was used to reveal global protein expression in chemosensitive breast cancer cells (MCF-7/WT) and comparing with the chemoresistant (adriamycin and paclitaxel resistant) breast cancer cells (MCF-7/ADR and MCF-7/PAC).The proteins of interest in the gels were then identified using MALDI-TOF mass spectrometry.The result revealed 26 proteins where level of expression could be differentiated between chemosensitive and chemoresistant breast cancers.These were also validated by western blot and immunohistochemistry (IHC) studies.In addition, the role of adenosine binding cassette (ABC)transporter proteins in chemoresistance breast cancer cells was investigated.The in vitro experiments have demonstrated an over-expression of P-glycoprotein (P-gp) in breast cancer cells resistant to adriamycin and paclitaxel, but not in chemosensitive cells.These findings have offered new insights in using these candidate proteins as predictors of response to chemotherapy in breast cancer, in particular in the neoadjuvant setting.