【摘 要】
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The newly discovered iron transporter,divalent metal transporter 1 (DMT1),is widely expressed in the brain.Its abnormal expression is related to the iron ac
【机 构】
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Department of Physiology, Medical College of Qingdao University, Qingdao 266071, China
【出 处】
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中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
The newly discovered iron transporter,divalent metal transporter 1 (DMT1),is widely expressed in the brain.Its abnormal expression is related to the iron accumulation of some neurodegenerative diseases.The aim of the present study is to investigate the effect of two forms of recombinant human DMT1 (with IRE and without IRE) expression on iron accumulation in glioma C6 cells.Results showed that recombinant adenovirus carrying human DMT 1+IRE and DMT 1-IRE gene could infect C6 cells efficiently.RT-PCR and Western Blotting confirmed the recombinant DMT1 expression in C6 cells.DMT1+IRE and DMT1-IRE could transport iron and induce the intracellular iron accumulation.Analysis of DNA extracted from Ad-DMT1 +IRE and Ad-DMT 1-IRE infected cells showed the typical "ladder pattern",indicating the formation of mono-and oligonucleosomes.
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