【摘 要】
：
林可霉素作为一种强效的抑菌性抗生素,因其对组织和细胞的穿透力强,在临床上被广泛使用.林可霉素A结构中存在着三个甲基,分别由C-、N-、S-甲基转移酶LmbW、LmbJ和LmbG所编码,
【机 构】
：
安徽大学健康科学研究院生命科学学院,合肥230601上海交通大学微生物代谢国家重点实验室,上海200240
论文部分内容阅读
林可霉素作为一种强效的抑菌性抗生素,因其对组织和细胞的穿透力强,在临床上被广泛使用.林可霉素A结构中存在着三个甲基,分别由C-、N-、S-甲基转移酶LmbW、LmbJ和LmbG所编码,其生物合成过程中的副产物林可霉素B缺少了C-甲基,因此,甲基转移与供给能力对林可霉素的产生至关重要.本项目首先在林可链霉菌高产工业菌株中过表达三个甲基转移酶LmbW、LmbJ和LmbG,通过实时荧光定量PCR分析发现,与空载菌株相比,过表达菌株自身基因的转录分别提高了6.0,5.8和2.5倍,但林可霉素产量提高不显著.
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