IMPROVED GLUTATHIONE PRODUCTION BY THE ADENOSINE DEAMINASE GENE DELETION OF ESCHERlCHIA COLI IN THE

• 作 者: JunLin[1]XianyanLiao[1]GuochengDu[1]JianChen[2]
• 机 构: School of Biotechnology, Jiangnan University, Wuxi 214112, CHINA
• 出 处: 第十四届世界食品科技大会
• 发表日期: 2008年期

　　Glutathione (γ-glutamyl-L-cysteinylglycine,GSH) is the most abundant free thiol compound in cells.GSH is now widely used in food industry as functional food ingredient.The requirement for ATP in the enzymatic production of GSH makes the production difficult to scale-up,because it is economically unfeasible to add ATP directly on an industrial scale.In our previous study,a coupled ATP regeneration system for GSH production was constructed with Escherichia coli JM109 (pBV03) and Saccharomyces cerevisiae WSH2,the former for GSH synthesis and the latter for ATP regeneration.