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Summary: Small non-coding RNAs (sRNAs),including microRNA (miRNA),piwi-interaeting RNAs (piRNAs) and siRNA et al.,have attracted considerable attention as an emerging class of gene expression regulators.As the length of sRNAs are.relatively short,such as miRNAs,which size are ranging from 18 to 24 nucleotides (nts),and piRNAs As with size ranging from 26 to 32-nt,cause hard to design primers for detection of sRNAs.