【摘 要】
：
甘蔗黄叶病(Sugarcane Yellow Leaf Disase,SCYLD)又称甘蔗黄叶综合症(Sugarcane Yellow Leaf Syndrome,SCYLS),是我国乃至世界甘蔗生产潜在的重要性病害.其病原是甘蔗黄叶病毒(Sugarcane Yellow Leaf Virus,SCYLV),属黄症病毒科(Luteoviridae)马铃薯卷叶病毒属(Poleroviruses).根
【机 构】
：
中国热带农业科学院热带生物技术研究所,农业部热带作物生物学与遗传资源利用重点实验室,海口571101;雅安职业技术学院,雅安625000
【出 处】
：
2014年度中国热带作物学会遗传育种专业委员会/热带香料饮料作物专业委员会年会暨学术研讨会
论文部分内容阅读
甘蔗黄叶病(Sugarcane Yellow Leaf Disase,SCYLD)又称甘蔗黄叶综合症(Sugarcane Yellow Leaf Syndrome,SCYLS),是我国乃至世界甘蔗生产潜在的重要性病害.其病原是甘蔗黄叶病毒(Sugarcane Yellow Leaf Virus,SCYLV),属黄症病毒科(Luteoviridae)马铃薯卷叶病毒属(Poleroviruses).根据甘蔗黄叶病毒海南分离物(ScYLV-CHN-HNl)全基因组序列(GenBank no.HQ342888)的P0蛋白基因设计一对特异性引物P0-BaitF/P0-BaitR.以实验室保存的质粒pMD 18T-P0为DNA模板,PCR扩增P0蛋白基因.将P0蛋白基因和细菌双杂交诱饵质粒pBT分别进行双酶切后,连接构建重组诱饵质粒pBT-P0.经PCR扩增、双酶切鉴定和序列分析,表明成功获得了细菌双杂交重组诱饵载体.进一步将重组载体pBT-P0转化细菌双杂交系统BacterioMatchR Ⅱ Screening Reporter Competent cells感受态细胞,并进行自激活和毒性验证.结果显示pBT-P0重组载体在细菌双杂交系统中无自激活及毒性作用,表明pBT-P0可应用于细菌双杂交系统,寻找与P0互作的甘蔗寄主蛋白.
其他文献
The progress and challenges of alternative technologies to animal testing in cosmetics safety evaluation The 7th Amendment to the cosmetics directive : Ban on animal testing in the EU.2004 Cosmetic pr
William Russell and Rex Burchs marvellous book, The Principles of Humane Experimental Technique,which was published in 1959.They emphasised the value of the Three Rs, which has become an important cri
目的:根据OECD(经合组织)测试指南439规定的方法,来验证使用EpiSkin表皮模型(中国生产)的皮肤刺激性体外测试方法的可实施性和结果的可靠性.国家质检总局的四个直属单位:检科院、北京局、上海局和广东局,以及欧莱雅中国研发中心共同参与了此次验证研究项目,是为在中国建立皮肤刺激性体外替代测试方法的相关标准提供科学的依据和认证的数据基础.材料和方法:此次研究采用的测试方法是根据EVCAM(欧洲体
目的:体外替代实验是化妆品毒性预测的重要手段,对于活性原料开发和配方研究也具有显著促进作用.在法规、技术和社会的三重压力下,检测机构和化妆品企业应当建立不以动物实验为中心的测试策略.本系列研究根据检测机构服务于产品质量评估和提供技术支撑的特点,尝试建立化妆品、化学品毒性测试的整合策略,提供细胞毒性/急性毒性预测、眼刺激性/腐蚀性、皮肤腐蚀/刺激性、皮肤光毒性、皮肤致敏等多毒性终点组合测试法,以及建
Phytochemical analysis of the high quality Chinese agarwood Qi-Nan originating from Aquilaria sinensis (Lour.) Glig, led to the isolation of four fragrant sesquiterpenes, including an agarofuran 4-hyd
A new carbazole alkaloid, claulansine K (1), together with six known carbazole alkaloids (2-7), was isolated from the peels of Clausena lansium (Lour.) Skeels.The new compound was elucidated using a c
Phytochemical analysis of the high quality Chinese agarwood Qi-Nan originating from Aquilaria sinensis (Lour.) Glig led to the isolation of a new 2-(2-phenylethyl) chromone derivative, qinanones G (1)
Five new sesquiterpenes (1 5) along with ten known ones were isolated from the heartwood of Dalbergia odorifrea T.Chen.Their structures were determined by spectroscopic techniques including MS, UV, IR
the number of laticifer cells in bark is one of the most important factors determining the biosynthesis and economic value of rubber trees (Hevea brasiliensis), the differentiation of laticifer cells
Six new steroidal saponins, cambodianosides A-F (1-6), together with seven known ones, were isolated from the dragons blood of Dracaena cambodiana.The structures of 1-6 were elucidated on the basis of