A Nvel Nno-bilayer Cllagen/Citosan Cmposite Mmbrane for Gided Bne Rgeneration

来源 :2014xi`an Conference of International Society for Maxillofac | 被引量 : 0次 | 上传用户:wh104311
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  Purpose : The purpose of present study was to develop a bi-layer membrane.Type Ⅰ collagen/chitosan/poly(ethylene oxide)nanofibrous film with porous allowing cells adhesion and proliferation;Collagen/chitosan film was on the opposite surface with freeze-dried for inhibition cells invasion.Methods: Chitosan or collagen dissolved in 1%(v/v)HAc solutions to prepare a 2%and 0.5%(w/v)solution,respectively.After removing entrapped air-bubbles,the mixed solution frozen at-20℃、-40℃、-80℃ for several hours,lyophilized for 24h to obtain a porous membrane.
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