【摘 要】
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Molecular imprinting is a well established and facile separation technique to produce molecule-specific recognition sites in synthetic polymers that selectively bind template molecules.For protein imp
【机 构】
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Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Tech
【出 处】
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第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
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Molecular imprinting is a well established and facile separation technique to produce molecule-specific recognition sites in synthetic polymers that selectively bind template molecules.For protein imprinting, surface polymerization has been proposed to prepare the MIPs layer on a solid support substrate, which can improve mass transfer and reduce permanent entrapment of the template.Metal coordination has been used as monomer in the preparation of protein MIP recently, since interactions between biological molecules and metal ions tend to be highly specific and usually reversible under mild reaction conditions.In our work, template protein Hemoglobin were immobilized on the surface of silica firstly, metal ions were used as an anchor points for the immobilization of protein during the formation of imprinted layer, then removed together with protein by EDTA.Template protein Hb was removed by formic acid.
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