【摘 要】
：
The process in which a target protein is directly captured from the crude feedstock, such as bacteria, yeast, and mammalian cell culture, is becoming important in biotechnology and pharmaceutical indu
【机 构】
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Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Coll
【出 处】
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第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
The process in which a target protein is directly captured from the crude feedstock, such as bacteria, yeast, and mammalian cell culture, is becoming important in biotechnology and pharmaceutical industry.Capture is usually performed by using liquid chromatography or batch adsorption.Adsorbent is the key in these processes.Therefore, to design and prepare new adsorbents with high performance is always a hot topic in this field.Surface-initiated atom transfer radical polymerization (SI-ATRP) is a new method for surface modification, which has been increasingly applied for preparation of adsorbent materials.This method has the advantage of excellent control over the molecular weight, polydispersity, and chain-end functionality of the grafted polymer.It also provides very satisfactory results in terms of the amount of grafted polymer.In this work, poly(chloromethylstyrene-co-divinylbenzene) beads were used as a macroinitiator, tert-butyl methacrylate (TBMA) was grafted onto the beads via SI-ATRP, then the grafted TBMA was hydrolyzed to produce a cation exchange absorbent.Parameters in SI-ATRP, such as solvent, reaction time, temperature, were investigated in detail.Adsorption isotherm for protein of each prepared absorbent was measured in batch mode, with lysozyme as a model protein, and the results showed that all the isotherms followed Langmuir equation.Adsorption conditions, such as equilibration time, salt concentration, buffer pH, additives, etc.were also investigated.The results showed that the prepared cation exchange adsorbent has very high adsorptive capacity for lysozyme.
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