【摘 要】
：
The advent of high-throughput approaches has revealed that a large portion of the mammalian genome is transcribed into long noncoding RNAs (IncRNAs).While many known IncRNAs are polyadenylated, recent
【机 构】
：
Shanghai Institutes for Biological Sciences,Chinese Academy of Science
【出 处】
：
8th IUPAP International Conference on Biological Physics(第八届
论文部分内容阅读
The advent of high-throughput approaches has revealed that a large portion of the mammalian genome is transcribed into long noncoding RNAs (IncRNAs).While many known IncRNAs are polyadenylated, recent work has revealed that a number of IncRNAs are processed in alternative ways without poly(A) tails, which largely failed to be detected by oligo(dT)purification.We have developed an enrichment strategy to purify non-polyadenylated (poly(A)-/ribo-) RNAs used to high throughput sequencing, this strategy has been successfully applied to identify the rich repertoire of non-polyadenylated RNAs in vivo,including a new class of nuclear-enriched intron-derived long noncoding RNAs that are processed on both ends by the snoRNA machinery (sno-lncRNAs).Importantly, the genomic region encoding one abundant class of sno-lncRNAs (15q11-q13)is specifically deleted in Prader-Willi Syndrome (PWS).
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