【摘 要】
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Glycerol dehydrogenases are key enzymes in the pathway of glycerol metabolism.They dehydrogenate the glycerol to dihydroxyacetone (DHA) for the production of value-added products,namely,DHA,butanol,su
【机 构】
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Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xia
【出 处】
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第七届中国工业生物技术发展高峰论坛
论文部分内容阅读
Glycerol dehydrogenases are key enzymes in the pathway of glycerol metabolism.They dehydrogenate the glycerol to dihydroxyacetone (DHA) for the production of value-added products,namely,DHA,butanol,succinic acid and citric acid.The substitution of the catalytic zinc ion of glycerol dehydrogenase (GDH) from Klebsiella pneumoniae sp by divalent metal ion,Mn2+,achieved an improvement of activity.A kinetic model based on ordered Bi- Bi mechanism was proposed considering the non-competitive product inhibition of DHA and the competitive product inhibition of NADH.The simulated values of time-concentration curves fitted the experimental values well with an average relative error of 11.5% and 12.7% for Mn-GDH and GDH,respectively.The kinetic parameters of GDH and Mn-GDH were estimated using model fitting.
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